X-Light V3

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Full compatibility
High-sectioning, High-speed
Enhanced sensitivity and image quality
Dual-camera ultra-large field of view
V3 CrestOptics
V3 CrestOptics

Description

X-Light V3 is the next generation of X-Light spinning disk confocal series.

It relies on the cutting-edge technology, advanced optical design approach and engineering solutions developed by CrestOptics to meet the very high-end specifications required by most of the modern fluorescence microscopy applications.

The spinning disk design together with the careful design of the optical layout enhances sensitivity and image clarity.

The first confocal unit which allows dual camera imaging at the full field of view of 25 mm on both cameras.

V3 Features insight

CrestOptics_V3Features_PinholeDisk01CrestOptics_V3Features_PinholeDisk02

X-Light Spinning Disk

The 50 um spinning disk is available in two different versions: standard spacing and larger spacing.

Standard X-Light spinning disk is the excellent compromise between brightness, confocality and image quality. The version with larger spacing has been designed to enhance even more the image quality and sectioning in thicker specimens thanks to the large reduction of crosstalk between pinholes.

Overlay image

Showing the Spinning Disk pinholes spacing in standard version (red) and larger spacing (green). Image acquired using a 4.2 Megapixel sCMOS camera

Visit our Publications page to explore a collection of scientific articles, where our cutting-edge microscopy instruments have been utilized, enabling significant discoveries across various research fields

Dual color, dual imaging, still wider

The first confocal unit which allows dual-camera imaging at the maximum field of view of 25 mm on both cameras.

External regulations allow to easily align the second camera with the first one

V3 CrestOptics

Image Gallery

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Illumination shaping

A software controlled square iris in front of the spinning disk, with variable aperture, can be used to screen light and preserve the surrounding area of the sample out of the region of interest.

 

Sealed Spinning disk box

This unique new feature makes the Spinning disk fully isolated and protected from environmental dust and prevents undesired artifacts related to the presence of small dust particles on the Spinning disk surface

Spinning disk synchronization

To manage very high-speed imaging, hundreds of fps, V3 provides as a standard feature a synchronization signal from the spinning disk to the camera.

A good way to make a solid quantitative analysis of very fast dynamics

Evolution through compatibility

DeepSim X-Light V3 CrestOptics

Combination with DeepSIM

Technical Features

Imaging modalitiesWidefield/Confocal/Super-resolution in combination with
DeepSIM X-Light
Compatible microscopesFull range upright and inverted microscopes
FOVUp to 25 mm diagonal
LaserMulti mode lasers with SMA coupling
Spectral rangeExcitation 400-750 nm/ Emission 400-850 nm
CameraCompatible with CCD/EMCCD cameras and sCMOS cameras
Dual-camera view: simoultaneous dual-camera imaging up to 25 mm
Disk speed/scan rate15000 RPM / > 1000 fps
Spinning disk geometry
(diameter/spacing)
50/250 μm pinholes for routine imaging
50/400 μm pinholes with wider spacing for deep imaging
Slit spirals for high-throughput applications
Customized disk available upon request
ResolutionLateral Resolution (FWHM): ~230 nm (High NA 1,4)
Axial Resolution (FWHM): ~600 nm (High NA 1,4)
Filter wheelsMotorized filter wheels and slider: 4-positions excitation filter wheel,
3-positions dichroic filter wheel, 8-positions emission filter wheel,
3 positions slider for dual camera use
SoftwareNIS Elements /μManager / MetaMorph/ Volocity / VisiView®
Recommended installation conditionsTemperature 23± 5°C, Humidity 70% RH or less (no condendation)
Weight26,0 Kg (57 lbs)
DimensionsX-Light V3:
370 (w) x 555.6 (l) x 226.5 (h) mm
14.5 (w) x 21.9 (l) x 8.9 (h) inches

X-Light V3 + DeepSIM X-Light:
370 (w) x 936.5 (l) x 290.5 (h) mm
14.5 (w) x 36.8 (l) x 11.4 (h) inches

 

Compatibility

List of compatible products

Detectors

Hamamatsu
Andor technology
PCO

Microscopes

Software

MetaMorph
NIS-Elements
MicroManager
Volocity
VisiView
Inscoper

For more information contact us

Distributors

CrestOptics relies on a large network of experienced and trained distributors worldwide who provide full commercial and technical support to laboratories and facilities

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