X-Light V2

X-Light V2

UNIVERSAL PLUG-IN SPINNING DISK
FOR FAST AND GENTLE IMAGING
Full compatibility
Plug-in spinning disk
Custom relay lenses
Wider field of view

Description

To guarantee each laboratory greater productivity without compromising the data quality, CrestOptics has created the X-Light V2, the most accessible spinning disk solution for fast and gentle confocal imaging.

The X-light V2 is a truly enabling technology where the high-performance is combined with the application fexibility and a universal compatibility with any upright and inverted microscopes with a camera port.

Heart Cell – Isolated myocyte imaged at 330 fps, calcium signal on the right

25mm field of view

Daphnia_V2 L-FOV_GreenDaphnia_V2-standard_Green-

The X-light V2 enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell dynamics with an acquisition speed of over 1000 fps on full FOV.

The multi-beam spinning method offers not only high-speed imaging but signicantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the X- light V2 the standard tool for 3D live cell imaging.

18mm brain organoid25mm brain organoid
neurons 18mm_RGBneurons 25mm_RGB

How you see

Technical Features

Spectral range (confocal/widefield): excitation: 400-750 nm; emission: 400-850 nm
Software controlled bypass mode: widefield to confocal
Fastest spinning disk in the market: 15.000 rpm rotation speed for artefact-free high speed acquisitions
Tailor-made disks: 50 um pinholes with two different pinole spacing. High throughput and custom pattern disks available on demand
Field of view: 25 mm
Exchangeable, synchronized and sealed: the disk box is one of the most versatile features of the new V3. Dust protection and synchronization signals out to the camera make it reliable and faster. Perfect for high speed acquisitions
Lateral Resolution (FWHM): ~230 nm (High NA 1.4) diffraction limited
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
Motorized filter wheels: 8-positions cleanup filter wheel (available on request), 5-positions dichroic filter wheel, 8-positions emission filter wheel

Learn More

Download Brochure
"The X-light V2 spinning disk enables us to routinely perform complicated live-imaging experiments for extended periods of time, using a variety of probes spanning dyes through genetically-encoded probes."
Professor David J. Hodson
Professor David J. HodsonInstitute of Metabolism and Systems Research (IMSR), University of Birmingham, Birmingham, UK.
Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UK.
Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK

Compatibility

List of compatible products

Detectors

Photometrics
Hamamatsu
Andor technology
PCO

Microscopes

Nikon
Olympus
Leica
Zeiss

Illuminators

89North – LDI

Lumencor – SPECTRA light engine

Lumencor – CELESTA light engine

CoolLED pE800

Excelitas Novem

Software

MetaMorph
NIS-Elements
MicroManager
Volocity
VisiView
Inscoper

For more information contact us

Distributors

CrestOptics relies on a large network of experienced and trained distributors worldwide who provide full commercial and technical support to laboratories and facilities

Where to buy