X-Light V2

UNIVERSAL PLUG-IN SPINNING DISK
FOR FAST AND GENTLE IMAGING
Full compatibility
Plug-in spinning disk
Custom relay lenses
Wider field of view
V2 CrestOptics
V2 CrestOptics

Description

To guarantee each laboratory greater productivity without compromising the data quality, CrestOptics has created the X-Light V2, the most accessible spinning disk solution for fast and gentle confocal imaging.

The X-light V2 is a truly enabling technology where the high-performance is combined with the application fexibility and a universal compatibility with any upright and inverted microscopes with a camera port.

Heart Cell – Isolated myocyte imaged at 330 fps, calcium signal on the right

25mm field of view

Daphnia_V2 L-FOV_GreenDaphnia_V2-standard_Green-

The X-light V2 enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell dynamics with an acquisition speed of over 1000 fps on full FOV.

The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the X- light V2 the standard tool for 3D live cell imaging.

18mm brain organoid25mm brain organoid
neurons 18mm_RGBneurons 25mm_RGB

Visit our Publications page to explore a collection of scientific articles, where our cutting-edge microscopy instruments have been utilized, enabling significant discoveries across various research fields

Image Gallery

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Technical Features

Imaging modalitiesWidefield/Confocal
Compatible microscopesFull range of upright a Compatible microscopes nd inverted microscopes
FOVUp to 25 mm diagonal
Light sourceLASER with 1,5 mm SMA | LED with 3 mm LLG
Spectral rangeExcitation 400-750 nm / Emission 400-850 nm
CameraCompatible with CCD/EMCCD cameras and sCMOS cameras
Disk speed/scan rate15000 RPM / > 1000 fps
Spinning disk geometry
(diameter/spacing)
50/250 μm pinholes optimized for LASER light source
60/220 μm pinholes optimized for LED light source
Slit spirals for high throughput & live imaging applications
Customized disk available upon request
ResolutionLateral Resolution (FWHM): ~230 nm (High NA 1.4)
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
Filter wheelsMotorized filter wheels: 8-positions excitation filter wheel, 5-positions
dichroic filter wheel, 8-positions emission filter wheel.
SoftwareNIS Elements / μManager / MetaMorph/ Volocity / VisiView®
Weight11.0 Kg (24.3 lbs)
Dimensions270 (w) x 325 (l) x 226.5 (h) mm
10.6 (w) x 12.8 (l) x 8.9 (h) inches

The product packaging has been certified by the International Safe Transit Association (ISTA).

Compatibility

List of compatible products

Detectors

Teledyne Photometrics
Hamamatsu
Andor technology
PCO

Microscopes

Nikon
Evident (former Olympus)
Leica
Zeiss

Illuminators

89North – LDI

Lumencor – SPECTRA light engine

Lumencor – CELESTA light engine

CoolLED pE800

Excelitas Novem

Software

MetaMorph
NIS-Elements
MicroManager
Volocity
VisiView
Inscoper
Neurolucida
Stereoinvestigator

For more information 

Distributors

CrestOptics relies on a large network of experienced and trained distributors worldwide who provide full commercial and technical support to laboratories and facilities

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