Webinars

Imaging Across Scale: A Guide to CrestOptics DeepSIM

03/22/2024

Register now to reserve your spot in this exciting webinar and begin your exploration into the realm of super-resolution imaging. Do not let this chance slip away!

Best practices from clearing methods to high content microscopy: exploring the 3D cellular complexity in depth

09/21/2023

Best practices from clearing methods to high content microscopy: exploring the 3D cellular complexity in depth.

Studying the three-dimensional (3D) cellular structure is crucial for gaining a more meaningful understanding of biological systems as it remarkably preserves the physiological characteristics of the cellular architecture. The study of 3D cellular structures has gained increasing significance in recent years due to advances in techniques such as clearing and microscopy. Together, these techniques provide researchers with the tools to deeply visualize complex 3D cellular architectures at high resolution, enabling detailed analysis of cellular structures and functions.

Expanding super-resolution applications with DeepSIM: unlocking hidden details in complex specimens

05/25/2023

Expanding super-resolution applications with DeepSIM: unlocking hidden details in complex specimens

The application of super-resolution microscopy is often limited to a narrow sample type such as 2D structures and thin tissue sections, and to the type of sample support that can be used for imaging, like microscopy slides and bottom-glass surfaces due to the rigid configuration and restricted performance of standard commercial systems. This is the reason CrestOptics has developed DeepSIM, the first super-resolution module that is compatible with any existing upright or inverted microscope with a camera port. It is as easy to use as a confocal microscope enabling scientists to access data from a large variety of samples.

Join us for an exciting webinar as we explore the revolutionary capabilities of DeepSIM super-resolution and how it can transform the field of biological imaging by enabling biologists to get meaningful super-resolved images in challenging samples. Led by our esteemed panel of experts, this webinar will feature real-world examples that demonstrate the transformative impact of DeepSIM across various biological domains. Furthermore, this webinar will go beyond theoretical concepts and will share valuable insights on optimizing imaging protocols, obtaining relevant training datasets, and harnessing the full potential of DeepSIM.

Imaging More – High content spinning disk acquisition and data analysis

09/23/2022

High Content Imaging, Spinning Disk, Automated Imaging and Analysis

In this joint webinar, we will:

  • a stable yet flexible platform for long-term live-cell high content that addresses common process bottlenecks to provide 24/7 operational efficiency.
  • fast confocal imaging of thick samples in 96well plate, such as spheroids, organoids, organ-on-a-chip, and tissues, with CrestOptics X-Light V3 spinning disk confocal system and long-range objectives.
  • NIS.ai deep learning software modules which can be trained to perform a number of different analysis tasks, such as segmentation, and can be integrated into an automated imaging and analysis pipeline.

New confocal solutions for 3D spatial transcriptomics

06/22/2022

Spatial Biology, Stitching, Disk customization

In this joint webinar, we will:

  • Spatial transcriptomics case-study in collaboration with Dr. Alvaro Crevenna, Head of Microscopy, EMBL Rome. Spatial transcriptomics experiments consist of sequential multi-markers imaging on large and complex samples and here we present the study of individual RNA molecules organization within a mouse brain section. This involves high-throughput and large-scale scanning of the sample, complex multidimensional acquisitions, including large area stitching, Z-stacks and multichannel imaging.
  • Zero-compromise approach: fast imaging with a four-camera setup. We present two different multi-camera setups for high-speed imaging, these solutions allow you to reach the highest acquisition speed while acquiring more than two channels simultaneously without giving up the wide 25 mm FOV.
  • Discover the advantages of the new HT-Slits disk for high throughput applications. Depending on the specimen brightness and thickness, it is crucial to match the disk pattern to the sample to optimize image quality and acquisition speed. Cestoptics offers the freedom to choose the most suitable spinning disk geometry according to the application and imaging requirements.

DeepSIM super-resolution: get the most out of your live cell imaging experience

02/07/2022

SuperResolution, DeepSIM, livingcells

In this joint webinar, we will:

  • SD and SR imaging are two complementary approaches to live cell imaging. Speed, wide field of view, high-resolution and advanced optical sectioning can be combined to achieve meaningful results from complex biological systems.
  • Only properly incubated live samples can guarantee reproducible results: Okolab will present the most suitable hardware and the best environmental conditions to run a spinning disk and super-resolution live imaging experiment.
  • The new SIM lighting discoveries are revolutionizing the old paradigms of super-resolution: DeepSIM guarantees long term-live cell imaging, fast speed acquisition and deep imaging to all researchers.

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