High Content Imaging, Spinning Disk, Automated Imaging and Analysis

In this joint webinar, we will:

• a stable yet flexible platform for long-term live-cell high content that addresses common process bottlenecks to provide 24/7 operational efficiency.
• fast confocal imaging of thick samples in 96well plate, such as spheroids, organoids, organ-on-a-chip, and tissues, with CrestOptics X-Light V3 spinning disk confocal system and long-range objectives.
• NIS.ai deep learning software modules which can be trained to perform a number of different analysis tasks, such as segmentation, and can be integrated into an automated imaging and analysis pipeline.

Spatial Biology, Stitching, Disk customization

In this joint webinar, we will:

• Spatial transcriptomics case-study in collaboration with Dr. Alvaro Crevenna, Head of Microscopy, EMBL Rome. Spatial transcriptomics experiments consist of sequential multi-markers imaging on large and complex samples and here we present the study of individual RNA molecules organization within a mouse brain section. This involves high-throughput and large-scale scanning of the sample, complex multidimensional acquisitions, including large area stitching, Z-stacks and multichannel imaging.
• Zero-compromise approach: fast imaging with a four-camera setup. We present two different multi-camera setups for high-speed imaging, these solutions allow you to reach the highest acquisition speed while acquiring more than two channels simultaneously without giving up the wide 25 mm FOV.
• Discover the advantages of the new HT-Slits disk for high throughput applications. Depending on the specimen brightness and thickness, it is crucial to match the disk pattern to the sample to optimize image quality and acquisition speed. Cestoptics offers the freedom to choose the most suitable spinning disk geometry according to the application and imaging requirements.

SuperResolution, DeepSIM, livingcells

In this joint webinar, we will:

• SD and SR imaging are two complementary approaches to live cell imaging. Speed, wide field of view, high-resolution and advanced optical sectioning can be combined to achieve meaningful results from complex biological systems.
• Only properly incubated live samples can guarantee reproducible results: Okolab will present the most suitable hardware and the best environmental conditions to run a spinning disk and super-resolution live imaging experiment.
• The new SIM lighting discoveries are revolutionizing the old paradigms of super-resolution: DeepSIM guarantees long term-live cell imaging, fast speed acquisition and deep imaging to all researchers.

In this joint webinar, we will:

• deepen the criticalities of volumetric imaging: what are the mistakes to avoid? How can speed be optimized? What are the best experimental designs to put in place?
• put these concepts into practice by walking through a complete live cell imaging experimental workflow from sample preparation, high-speed volumetric acquisitions, and image processing.
• present unpublished acquisitions of live zebrafish embryos: vascular tissue; migration of macrophage cells through the embryo and finally the localization of tumor cells inside the yolk sac.
• reveal Hydra vulgaris secretory organelle details in high resolution using high magnification objectives.