In this joint webinar, we will:

• deepen the criticalities of volumetric imaging: what are the mistakes to avoid? How can speed be optimized? What are the best experimental designs to put in place?
• put these concepts into practice by walking through a complete live cell imaging experimental workflow from sample preparation, high-speed volumetric acquisitions, and image processing.
• present unpublished acquisitions of live zebrafish embryos: vascular tissue; migration of macrophage cells through the embryo and finally the localization of tumor cells inside the yolk sac.
• reveal Hydra vulgaris secretory organelle details in high resolution using high magnification objectives.