Super resolution microscopy that answers the deep biological questions effortlessly
At CrestOptics, we believe that super-resolution should be accessible for all scientists to progress their research. This is the reason we have developed DeepSIM, the first super-resolution module that is compatible with any existing upright or inverted microscope with a camera port. It’s as easy to use as a confocal microscope enabling scientists to access deep data from their biological samples.
Confocal
upgrade
Stand-alone
super resolution
Deep
imaging
Did you miss the DeepSIM launch event?
Watch the event recording here.
Learn how to evolve your routine microscope to Super-Resolution (SR) effortlessly, reveal sub-organelle structures down to 100 nm, and make SR acquisition deeper than ever before.
Description
Based on a multi-spot structured illumination system, the DeepSIM is a reliable, simple to use and affordable solution to study cellular structures up to an XY resolution of ~100nm.
Super-resolved optical sectioning, with Z resolution up to ~ 300nm, can be obtained using both high (60X – 100X) and low magnification (20x – 40x) objectives to expand the range of applications to include complex 3D models such as tissues, organoids, spheroids and small organisms.
The DeepSIM is designed to work with samples of thicknesses comparable to those used in confocal microscopy, giving super-resolved data over 50µm Z in depth in non-clarified samples.
This means that more meaningful data can be obtained from native heterogeneous complex samples using routine preparation protocols.
DeepSIM enables the effortless study of live-cell dynamics through a temporal resolution greater than 10fps (1024×1024 px FOV), allowing biological changes to be tracked at cellular and subcellular levels.
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What you can see
How you see
Select your objective (click the buttons on the left)
Comparison of mouse primary microglia cell, alpha-tubulin.
| widefield | confocal | DeepSIM super-resolution |
Comparison of HeLa cells expressing GFP-alpha tubulin (green) and stained for lysosomes (red).
| widefield | confocal | DeepSIM super-resolution |
Comparison of cleared mouse intestine section (0.55 mm thickness) 25X Sil
| widefield | confocal | DeepSIM super-resolution |
Comparison of Hippocampal coronal slice from mouse brain
| widefield | confocal | DeepSIM super-resolution |
Evolution through compatibility
DeepSIM is designed to be a truly enabling technology where performance is combined with the flexibility of a system available either as a super-resolution module combined with CrestOptics X-Light V3 Spinning Disk or as stand-alone equipment.
DeepSIM stand-alone
Technical Features
| DeepSIM stand-alone | DeepSIM X-Light | |
| FOV | 1024x1024pixel (66×66 µm 100X | 333×333µm 20X) | 1024x1024pixel (66×66 µm 100X | 333×333µm 20X) |
| Resolution | Lateral Resolution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45) | Lateral Resolution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45) |
| DeepSIM Acquisition speed | 13fps (1024x1024px) | 13fps (1024x1024px) |
| Laser spectral range | Excitation: 400-750 nm; emission: 400-850 nm | Excitation: 400-750 nm; emission: 400-850 nm |
| Objective specifications | -from 20X to 100X magnification range -high numerical aperture (NA) -plan apochromat correction | -from 20X to 100X magnification range -high numerical aperture (NA) -plan apochromat correction |
| Camera compatibility | Any triggerable camera having 6.5 µm pixel size | Any triggerable camera having 6.5 µm pixel size |
| Multi cameras option | Single camera | Dual camera option available* |
| Spinning disk upgrade | Stand-alone solution | Add-on compatible with CrestOptics X-Light V3 |
| Imaging modalities | Super-resolution DeepSIM | Widefield | Super-resolution DeepSIM | Confocal spinning disk X-light V3 | Widefield |
| Upgradable microscope configuration | Upright and inverted configurations | Inverted configuration |
| Software | µManager /VisiView® / NIS Elements | µManager /VisiView® / NIS Elements |
| Installation Conditions | Temperature 23 ± 5°C, Humidity 70% RH or less | Temperature 23 ± 5°C, Humidity 70% RH or less |
| Weight | 50.7 lbs | 23Kg | 44 lbs | 20Kg |
| Dimensions | 13.8 (w) x 20.2 (L) x 11.4 (h) inches 352.0 (w) 514.0 (L) x 290.5 (h) mm | 14.0 (w) x 17.1 (L) x 11.4 (h) inches 356.0 (w) x 435.0 (L) x 290.5 (h) mm |
*Software integration in progress
Distributors
CrestOptics relies on a large network of experienced and trained distributors worldwide who provide full commercial and technical support to laboratories and facilities
