Super resolution microscopy that answers the deep biological questions effortlessly

At CrestOptics, we believe that super-resolution should be accessible for all scientists to progress their research. This is the reason we have developed DeepSIM, the first super-resolution module that is compatible with any existing upright or inverted microscope with a camera port. It’s as easy to use as a confocal microscope enabling scientists to access deep data from their biological samples.

super resolution

Did you miss the DeepSIM launch event?

Watch the event recording here.

Learn how to evolve your routine microscope to Super-Resolution (SR) effortlessly, reveal sub-organelle structures down to 100 nm, and make SR acquisition deeper than ever before.


Based on a multi-spot structured illumination system, the DeepSIM is a reliable, simple to use and affordable solution to study cellular structures up to an XY resolution of ~100nm.

Super-resolved optical sectioning, with Z resolution up to ~ 300nm, can be obtained using both high (60X – 100X) and low magnification (20x – 40x) objectives to expand the range of applications to include complex 3D models such as tissues, organoids, spheroids and small organisms.

The DeepSIM is designed to work with samples of thicknesses comparable to those used in confocal microscopy, giving super-resolved data over 50µm Z in depth in non-clarified samples.

This means that more meaningful data can be obtained from native heterogeneous complex samples using routine preparation protocols.

DeepSIM enables the effortless study of live-cell dynamics through a temporal resolution greater than 10fps (1024×1024 px FOV), allowing biological changes to be tracked at cellular and subcellular levels.

Learn More

What you can see​

How you see

Select your objective (click the buttons on the left)

Evolution through compatibility

DeepSIM is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system available either as Super Resolution add-on module for CrestOptics X-light V3 Spinning Disk system or standalone equipment.

Combination with spinnign-disk confocal

Stand-alone unit

Technical Features

FOV: 1024x1024pixel (66x66µm 100X | 333x333µm 20X)
Laser: Spectral range: excitation: 400-750 nm; emission: 400-850 nm
Camera: Compatible with any triggerable camera having 6.5 µm pixel size
Dual camera option: Available
Acquisition speed: 13fps (1024x1024px)
Spinning disk upgrade: Compatible with CrestOptics X-light V3
Objectives compatible: From 100X to 20X range
Resolution: Lateral Resolution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
Software: Micro-Manager / VisiView® / NIS elements
Recommended Installation Conditions: Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)
Weight: 44 lbs | 20Kg
Dimensions: 14.0 (w) x 17.1 (L) x 11.8 (h) inches | 356.0 (w) x 435.0 (L) x 300.0 (h) mm


List of compatible products


Andor technology





For more information contact us


CrestOptics relies on a large network of experienced and trained distributors worldwide who provide full commercial and technical support to laboratories and facilities