New confocal solutions for 3D spatial transcriptomics

Due to their capacity to allow both proteomics and transcriptomics profiling, spatial biology techniques are advancing rapidly as powerful tools to obtain an in-depth understanding of tissues. With the primary goal of creating complete maps of large and complex tissues by studying individual cells, these techniques represent a game-changer in learning about their function. Spatially resolved transcriptomics studies can be carried out by microscopy through, for example, in situ sequencing or multiplexed fluorescence in situ hybridization. CrestOptics X-light V3 spinning disk microscopy provides a robust solution for high-throughput single-cell resolution imaging for spatial biology analysis.

In this joint webinar, we will talk about:

  • Spatial transcriptomics case-study in collaboration with Dr. Alvaro Crevenna, Head of Microscopy, EMBL Rome. Spatial transcriptomics experiments consist of sequential multi-markers imaging on large and complex samples and here we present the study of individual RNA molecules organization within a mouse brain section. This involves high-throughput and large-scale scanning of the sample, complex multidimensional acquisitions, including large area stitching, Z-stacks and multichannel imaging.
  • Zero-compromise approach: fast imaging with a four-camera setup. We present two different multi-camera setups for high-speed imaging, these solutions allow you to reach the highest acquisition speed while acquiring more than two channels simultaneously without giving up the wide 25 mm FOV.  
  • Discover the advantages of the new HT-Slits disk for high throughput applications. Depending on the specimen brightness and thickness, it is crucial to match the disk pattern to the sample to optimize image quality and acquisition speed. Cestoptics offers the freedom to choose the most suitable spinning disk geometry according to the application and imaging requirements. 

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