High resolution imaging of 3D organoids at low magnification

Acquisition @ Scuola di Microscopia 2019, in collaboration with Crisel Instruments srl

Here are shown organoids cultured in Matrigel on optical multi-well plates and generated from primary stem cells from colon cancer biopsies and stained for cytoskeleton by Phalloidin-568.

Comparison between Widefield, Confocal Spinning Disk and Super Resolution acquisitions.

Widefield

X-Light V2 Confocal Spinning Disk

X-Light V2 Confocal Spinning Disk
imaging of 3d organoids
Video Confocal Super-resolution (VCS)
Primary stem cell sample courtesy of Chiara Cordiglieri
INGM Imaging Facility
Istituto Nazionale Genetica Molecolare (Milan)

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