Ventricle-like structure in human iPSC derived cortical organoid

Human iPSC (Induced Pluripotent Stem Cells) are able to generate cortical organoids in vitro after a specific differentiation protocol. Cortical organoids are self-organizing three-dimensional cultures that model features of the developing human cerebral cortex.

Here is shown a ventricle-like structure within a cortical organoid of 50 days in vitro, acquired with CrestOptics Confocal Spinning Disk and stained for MAP2 (cyan), Pax6 (red) and N-Cadherin (green), which are markers for ventricular and subventricular-like regions.