Ventricle-like structure in human iPSC derived cortical organoid

Human iPSC (Induced Pluripotent Stem Cells) are able to generate cortical organoids in vitro after a specific differentiation protocol. Cortical organoids are self-organizing three-dimensional cultures that model features of the developing human cerebral cortex.

Here is shown a ventricle-like structure within a cortical organoid of 50 days in vitro, acquired with CrestOptics Confocal Spinning Disk and stained for MAP2 (cyan), Pax6 (red) and N-Cadherin (green), which are markers for ventricular and subventricular-like regions.

X-Light V3 Confocal Spinning Disk acquisition (40x objective)

Zoom in (60x objective)

Human iPSC derived organoid courtesy of Alessandro SolopertoCarlo BrighiSilvia Di Angelantonio
Istituto Italiano di Tecnologia (IIT) CLNS@SAPIENZA Roma

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