ELMI 2024 in Liverpool

June 4-7, 2024

CrestOptics is pleased to announce our participation in the upcoming European Light Microscopy Initiative (ELMI) conference, taking place from 4 to 7 June 2024 in Liverpool, UK. We are looking forward to connecting with the microscopy community at ELMI 2024, which serves as a unique platform for European scientists in the field of light microscopy to engage with equipment manufacturers. ELMI’s mission is to advance the use of light microscopy as a vital research tool in the life sciences and to facilitate communication among researchers, core facilities, and industry.

Visit our booth to explore our state-of-the-art X-Light V3 spinning disk and DeepSIM super-resolution systems, which offer researchers the opportunity to easily achieve clear and detailed imaging for enhanced visualization of biological processes.

In addition, our specialists will present the three following WORKSHOPS, on stand #8 (please, note that to sign up for our workshops, visit the dedicated ELMI workshop webpage or click on the button below, capacities for each workshop are limited and the deadline for booking is 21 May 2024): 

1) From cells to organs with spinning disk confocal and SIM super-resolution imaging across scales

14:30 – 15:30 BST, 5 June 2024 ‐ 1 hour

Many microscopy facilities strive to offer versatile tools for diverse user needs, but often fall short. However, the CrestOptics X-Light V3 / DeepSIM platform is game-changer, bridging this gap. It excels in high magnification, fast multicolor live cell imaging, and massive 3D volumetric imaging, surpassing traditional microscopy limitations like slow acquisition and inadequate depth penetration. This workshop explores spinning disk and SIM super-resolution imaging in cutting-edge 3D biological research.

2) Correlative multimodal bioimaging: an holistic approach to investigate biological samples

15:40 – 16:40 BST, 5 June 2024 ‐ 1 hour

Correlative multimodal bioimaging combines different imaging technologies to enhance the understanding of biological samples. This workshop shows a correlative multimodal microscopy approach that exploits the major advantages of Spinning Disk Confocal and SIM super-resolution technologies to get the most out of a whole set of biological samples from different spatial scales.

3)  Imaging-based spatial -omics: merging Spinning Disk Confocal and SIM technologies from tissue to single cell level

17:10 – 18:10 BST, 5 June 2024 ‐ 1 hour

Spinning Disk Confocal and SIM have the capacity to improve spot detection and overall data quality in spatial transcriptomics with respect to standard widefield microscopy plus deconvolution approach.In particular in this workshop, it will be shown how Spinning Disk Confocal tile scan and Z-stacks of entire tissue sections are accomplished at high speed and how SIM super-res can help to untangle high-density areas and resolve numerous spots in close proximity at subcellular levels.

Come see live demonstrations at booth #8 and discover how CrestOptics can enhance your imaging capabilities and enable multimodal bioimaging across disciplines and scales.

To sign up for our workshops, please visit the dedicated ELMI workshop webpage. Capacities for each workshop are limited so act fast to secure your preferred options. The deadline for booking is 21 May 2024.

Imaging-based spatialomics: merging Spinning Disk Confocal and SIM super-res technologies from tissue to single cell level.

Left: Large image of a mouse brain section marked with different RNA species; nuclei are stained in blue. Objective 40x, 1.15 NA, Water immersion. The Maximum intensity projection of a 15 µm Z-stack, acquired with the X-Light V3 Spinning Disk Confocal on a Nikon Ti2 Eclipse inverted microscope, is shown. Image courtesy: Dr. Kwakwa, Wellcome Sanger Institute, UK. For the related Application Note, please click here.

Right: DeepSIM acquisition of a mouse brain section marked with four different RNA species (green, red, cyan and white dots) and nuclei in blue with DAPI. Objective 25X 1.05 NA Silicon oil immersion. Sample credits: Dr. Crevenna, EMBL Rome, Italy. For the related Application Note, please click here.

Cover image: dissociated neurons co-cocultured with cancer organoids. DeepSIM acquisition, 60x Oil, NA 1.42. DAPI for nuclei, EpCAM stains cancer cells in green, b3 Tubulin in red, SV2A (synaptic density marker) in orange, Synaptophysin (neuroendocrine presynaptic marker) in white. Sample credits: Prof. Hwang, Dr. Wang, Harvard University, MA, USA.

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