VCS Super resolutions model

VCS
Super resolutions model

VCS upgrade X-Light V2 to experience the full 3Dsuper-resolution of structured illumination microscopy

Confocal upgrade

Lateral super-resolution

Super-resolution sectioning

描述

VCS (VideoConfocal super-resolution) module is a new system developed as an add-on for the X-light V2.

Using an approach based on super-resolution patents from Pisa CNR (which assume the 2-dimensional scan of the sample with a pinhole pattern and several algorithms application), CrestOptics-VCS enhance 3D resolution of the standard light microscope.

Parallel calculation is implemented exploiting CUDA technology for a very fast processing time.

The full system X-Light V2 + VCS is thought to work in three configurations:

Widefield mode
Confocal mode
Super-resolution mode

CrestOptics has been admitted to the following tender:

“BREVETTI +” SOTTOMISURA 2 “INCENTIVI PER LA VALORIZZAZIONE ECONOMICA DEI BREVETTI”

Public Announcement dated August 3, 2011

Protocol N°5002159 – COD. DOMANDA 3717EC submitted on June 23, 2014

CUP code: C84E15000260008

Project title: “Metodo per la raccolta di immagini in microscospia confocale”

Contract stipulated with Invitalia on December 23, 2015

您所看到的样子

Comparison of Hela Cells Interphase in standard resolution vs VCS super-resolution

技术特点

Software controlled bypass mode for widefield, confocal and super-resolution

Up to 18 mm field of view

Lateral resolution ~120 nm*

Axial resolution ~300 nm*

Highly corrected immersion objectives compatible

60x
100x

Suitable for thick specimen imaging < 100 um

Time resolution: ~ 5 sec/frame

Camera specification:6.5 µm pixel size camerasCMOS QE>80%, low readout noise ≤ 1e- strongly recommended

Camera specification:

6.5 µm pixel size camera
sCMOS QE>80%, low readout noise ≤ 1e- strongly recommended

Video Card/CUDA specifications:

Reccomended requirments:

Compute capability version: 5.0 or higher
GPU Memory: 4 Gb or higher

Link to compute capability versions and video cards CUDA supported:

https://developer.nvidia.com/cuda-gpus

PC/Workstation RAM 16 Gb or higher

Elaboration speed depends on GPU performances

* Dependant on optics properties, objectve magnification and camera pixel size

VCS/SCSR pixel calibration table*

Camera pixel size (Magnification)	6.5 µm	11 µm	13 µm
60x	54.2 nm/pixel	91.6 nm/pixel	108 nm/pixel
100x	32.5 nm/pixel	55 nm/pixel	65 nm/pixel

*Pixel calibration after using Super-Confocal/Super-Resolution VCS algorithm. A 6.5 µm pixel camera must be used to have the required sampling for maximum lateral resolution.