VCS
Super resolutions model

VCS upgrade X-Light V2 to experience the full 3Dsuper-resolution of structured illumination microscopy
Confocal upgrade
Lateral super-resolution
Super-resolution sectioning

描述

VCS (VideoConfocal super-resolution) module is a new system developed as an add-on for the X-light V2.

Using an approach based on super-resolution patents from Pisa CNR (which assume the 2-dimensional scan of the sample with a pinhole pattern and several algorithms application), CrestOptics-VCS enhance 3D resolution of the standard light microscope.

Parallel calculation is implemented exploiting CUDA technology for a very fast processing time.

The full system X-Light V2 + VCS is thought to work in three configurations:

  • Widefield mode
  • Confocal mode
  • Super-resolution mode

CrestOptics has been admitted to the following tender:

“BREVETTI +” SOTTOMISURA 2 “INCENTIVI PER LA VALORIZZAZIONE ECONOMICA DEI BREVETTI”

Public Announcement dated August 3, 2011

Protocol N°5002159 – COD. DOMANDA 3717EC submitted on June 23, 2014

CUP code: C84E15000260008

Project title: “Metodo per la raccolta di immagini in microscospia confocale”

Contract stipulated with Invitalia on December 23, 2015

您所看到的样子

Comparison of Hela Cells Interphase in standard resolution vs VCS super-resolution

HeLa cellsHeLa cells

技术特点

Software controlled bypass mode for widefield, confocal and super-resolution
Up to 18 mm field of view
Lateral resolution ~120 nm*
Axial resolution ~300 nm*
Highly corrected immersion objectives compatible
  • 60x
  • 100x
Suitable for thick specimen imaging < 100 um
Time resolution: ~ 5 sec/frame
Camera specification:6.5 µm pixel size camerasCMOS QE>80%, low readout noise ≤ 1e- strongly recommended
Camera specification:
  • 6.5 µm pixel size camera
  • sCMOS QE>80%, low readout noise 1e- strongly recommended

Video Card/CUDA specifications:

Reccomended requirments:
  • Compute capability version: 5.0 or higher
  • GPU Memory: 4 Gb or higher
Link to compute capability versions and video cards CUDA supported:
PC/Workstation RAM 16 Gb or higher
Elaboration speed depends on GPU performances
* Dependant on optics properties, objectve magnification and camera pixel size

VCS/SCSR pixel calibration table*

Camera pixel size
(Magnification)
6.5 µm11 µm13 µm
60x54.2 nm/pixel91.6 nm/pixel108 nm/pixel
100x32.5 nm/pixel55 nm/pixel65 nm/pixel
*Pixel calibration after using Super-Confocal/Super-Resolution VCS algorithm. A 6.5 µm pixel camera must be used to have the required sampling for maximum lateral resolution.

兼容性

能兼容产品

Detectors

Photometrics
Hamamatsu
Andor technology
PCO

Microscopes

Nikon
Olympus
Leica
Zeiss

Software

MetaMorph
NIS-Elements
MicroManager
Volocity
VisiView

For more information contact us

经销商

CrestOptics仰赖在世界各地经验丰富且训练有素的经销商网络,来提供全面商务和技术支持给实验室和机构。