VCS
Super resolutions model

VCS upgrade X-Light V2 to experience the full 3D super-resolution of structured illumination microscopy
Confocal upgrade
Lateral super-resolution
Super-resolution sectioning

Description

VCS (VideoConfocal super-resolution) module is a new system developed as an add-on for the X-light V2.

Using an approach based on super-resolution patents from Pisa CNR (which assume the 2-dimensional scan of the sample with a pinhole pattern and several algorithms application), CrestOptics-VCS enhance 3D resolution of the standard light microscope.

Parallel calculation is implemented exploiting CUDA technology for a very fast processing time.

The full system X-Light V2 + VCS is thought to work in three configurations:

  • Widefield mode
  • Confocal mode
  • Super-resolution mode

CrestOptics has been admitted to the following tender:

“BREVETTI +” SOTTOMISURA 2 “INCENTIVI PER LA VALORIZZAZIONE ECONOMICA DEI BREVETTI”

Public Announcement dated August 3, 2011

Protocol N°5002159 – COD. DOMANDA 3717EC submitted on June 23, 2014

CUP code: C84E15000260008

Project title: “Metodo per la raccolta di immagini in microscospia confocale”

Contract stipulated with Invitalia on December 23, 2015

How you see

Comparison of Hela Cells Interphase in standard resolution vs VCS super-resolution

HeLa cellsHeLa cells

Technical Specifications

Software controlled bypass mode for widefield, confocal and super-resolution
Up to 18 mm field of view
Lateral resolution ~120 nm*
Axial resolution ~300 nm*
Highly corrected immersion objectives compatible
  • 60x
  • 100x
Suitable for thick specimen imaging < 100 um
Time resolution: ~ 5 sec/frame
Camera specification: 6.5 µm pixel size camera sCMOS QE>80%, low readout noise ≤ 1e- strongly recommended
Camera specification:
  • 6.5 µm pixel size camera
  • sCMOS QE>80%, low readout noise 1e- strongly recommended

Video Card/CUDA specifications:

Reccomended requirments:
  • Compute capability version: 5.0 or higher
  • GPU Memory: 4 Gb or higher
Link to compute capability versions and video cards CUDA supported:
PC/Workstation RAM 16 Gb or higher
Elaboration speed depends on GPU performances
* Dependant on optics properties, objectve magnification and camera pixel size

VCS/SCSR pixel calibration table*

Camera pixel size
(Magnification)
6.5 µm11 µm13 µm
60x54.2 nm/pixel91.6 nm/pixel108 nm/pixel
100x32.5 nm/pixel55 nm/pixel65 nm/pixel
*Pixel calibration after using Super-Confocal/Super-Resolution VCS algorithm. A 6.5 µm pixel camera must be used to have the required sampling for maximum lateral resolution.

Compatibility

List of compatible products

Detectors

Photometrics
Hamamatsu
Andor technology
PCO

Microscopes

Nikon
Olympus
Leica
Zeiss

Software

MetaMorph
NIS-Elements
MicroManager
Volocity
VisiView

For more information contact us

Distributors

CrestOptics relies on a large network of experienced and trained distributors worldwide who provide full commercial and technical support to laboratories and facilities