Mitotic spindle in acute myeloid leukemia cells

Acquisition @ Scuola di Microscopia 2019, in collaboration with Crisel Instruments srl

Acute myeloid leukemia (AML) is a heterogeneous disease in which a variety of distinct genetic alterations might occur and genetic instability plays an important role.

Proper mitotic control is a prerequisite to guarantee the equal distribution of the genetic material onto the two developing daughter cells, therefore the study of mechanisms which orchestrate cell division, such as mitotic spindle assembly, is relevant to fully understand the onset and progression of acute myeloid leukemia.

Here a hematopoietic mitotic cell CD34+ is shown. Cells were isolated from acute myeloid leukemia patient and stained for centrosomal and mitotic spindle markers (pericentrin is shown in red and g-tubulin green).

X-Light V2 Confocal Spinning Disk acquisition

Pericentric intensity profile detected along the yellow arrow.

Video Confocal Super-resolution (VCS) acquisition

Pericentric intensity profile detected along the yellow arrow.

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