Imaging a living Zebrafish

Acquisition @ Scuola di Microscopia 2019, in collaboration with Crisel Instruments srl

Transgenic Zebrafish (4 days old) expressing fluorescent proteins: lyn-TagRFP, neuron cellular membrane marker, and nls-GCaMP6s, calcium sensor in the nucleus. Fluorescent calcium sensors are widely used to image neural activity and GCaMP6 sensors are involved in the organization and dynamics of neural circuits over multiple spatial and temporal scales. The transgenic animal shown here (Maximum intensity Z-projection) was acquired with CrestOptics Confocal Spinning Disk.

X-Light V2 Confocal Spinning Disk acquisition

Autoquant 3D Deconvolution (theoretical PSF) and zoomed inset shown

3D movie of deconvolved image

Transgenic animal sample courtesy of Marco Dal Maschio

Department of Biomedical Sciences, University of Padua

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